Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the zymogen forms of the C1s protease is activated using immobilized recombinant protease C1r | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type enzyme and K628Q and K628A mutants in Escherichia coli as insoluble proteins in inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K628A | the mutant shows lower activity than wild-type enzyme against peptide substrates, cleavage of C4 is reduced by 5fold compared to the wild-type enzyme | Homo sapiens |
K628Q | the mutant shows greater activity than wild-type enzyme against peptide substrates and peptide substrates containing physiological substrate sequences, unaltered activity with 2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 substrate compared to the wild-type enzyme, but cleavage of C4 complement is reduced by 5fold compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C1-inhibitor | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
0.0123 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
0.0138 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.0654 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
0.0762 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
0.109 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
0.169 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.212 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
0.245 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09871 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the zymogen form of the C1s protease is activated using immobilized recombinant protease C1r | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant refolded and solubilized wild-type enzyme and K628Q and K628A mutants from Escherichia coli by anion exchange chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 + H2O | the Abz-GLQRALEI-Lys(Dnp)fluorescence quenched peptide representing the P4-P4' sequence of complement C4 | Homo sapiens | ? | - |
? | |
C4 complement + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | substrate specificity, library screening, overview. The Lys residue found at the P1' position in C2 and the Gln residue found at the P2 position of C4 are likely to be prohibitive for cleavage of substrates by the C1s protease. The K628Q and K628A mutants of C1s also do not cleave these substrate pools, indicating that K628 does not play a crucial role in preventing interaction with such substrates | Homo sapiens | ? | - |
? | |
Z-AGLQR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-AGLQR + 7-amino-4-methylcoumarin | - |
? | |
Z-LGR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-LGR + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C1s | - |
Homo sapiens |
C1s protease | - |
Homo sapiens |
complement protease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
1.17 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
1.3 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
1.3 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
1.77 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
2.72 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
6 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
35.3 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
116.5 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
12 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
16 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
79 | - |
Z-LGR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
82 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
95 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens | |
110 | - |
2-aminobenzoyl-GLQRALEI-Lys(dinitrophenol)-NH2 | pH 7.4, 37°C, recombinant mutant K628A | Homo sapiens | |
140 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
1800 | - |
Z-AGLQR-7-amido-4-methylcoumarin | pH 7.4, 37°C, recombinant mutant K628Q | Homo sapiens |